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Reconstitute 2 mL BAC water Concentration 2,500 mcg/mL per peptide Daily draw 8 units = 0.08 mL = ~200 mcg of each Typical cycle 12-16 weeks Dose selector Pre-Blended CJC/Ipa Combo Vial The combo vial removes one reconstitution but locks in a fixed 1:1 ratio

To obtain additional restraints for structural model determination of the p53 TAD2 -FOXO4 FH complex, we determined paramagnetic relaxation enhancements (PREs) for a set of 7 complexes containing spin-labeled (SL) samples ( 15 N-labeled FOXO4 FH + S46C-SL p53 TAD2 , 15 N-labeled FOXO4 FH + Q52C-SL p53 TAD2 , 15 N-labeled p53 TAD2 + Q112C-SL FOXO4 FH , 15 N-labeled p53 TAD2 + D140C-SL FOXO4 FH , 15 N-labeled p53 TAD2 + N145C-SL FOXO4 FH , 15 N-labeled p53 TAD2 + S159C-SL FOXO4 FH , 15 N-labeled p53 TAD2 + S162C-SL FOXO4 FH )

Cell viability was quantified using the Cell Counting Kit-8 (CCK8) assay

Jonathann Kuo, MD