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Horseradish peroxidase-conjugated secondary antibodies were diluted 1:10,000 and incubated for 1 h, and chemiluminescence solution and an electrochemiluminescence (ECL) kit (Millipore, USA) were used to detect protein band
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oxysporum infection was markedly increased (Han et al., 2016)
Step 6: Insert and Press the Injection Once you locate the site, just pinch it and insert the injection needle inside
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