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After 24 h, treated biofilm supernatant was washed once with 1 PBS followed by addition of 200 L of 1 PBS plus 15 L resazurin 0.05% w/v solution, and incubated for a further 24 h, at 37C, 150 rpm
even small temperature excursions accumulate across weeks of active use, and careful logging makes it straightforward to correlate experimental drift with material handling
6, A388A389
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