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Under physiological conditions, several modulators interact to maintain normal blood pressure, including the renin-angiotensin system (RAS), sympathetic nervous system, natriuretic peptide, sodium excretion, and endothelial (11)

The MSIA tips are then rinsed with a series of buffer and water rinses to remove salts and detergents ( Figure 1 , step 3), after which captured IGF-1/LR3 are eluted from the MSIA tips by iteratively pipetting an elution solvent of 33% acetonitrile in 0.4% trifluoroacetic acid, TFA (step 4)

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