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The pellet was resuspended in 200 l of 8 M urea with 10 mM Tris (pH 8), incubated at 60 C for 30 min with shaking and centrifuged for 15 min at 18,000 g for protein quantification of the supernatant with the DC Protein Assay (Bio-Rad Laboratories)
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Animal and test-tube studies indicate that they also affect tissue repair and wound healing ( Chapped lips are a common symptom of deficiencies, especially in folate (vitamin B9), riboflavin (vitamin B2), and vitamins B6 and B12 ( People with disorders that affect nutrient absorption such as celiac disease, chronic gastritis, and Crohns disease are especially susceptible to deficiencies ( Given that vitamin B12 is found primarily in animal products, vegans and vegetarians may also be at a higher risk of deficiency ( Furthermore, a deficiency in B vitamins may lead to dermatitis, depression, irritability, and fatigue ( summary Deficiencies in multiple nutrients, including iron, zinc, and B vitamins, can cause chapped lips
