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Oxidant resistance measurement We investigated whether FreSHtracer-isolated hES-MSCs have enhanced resistance to oxidative stress by treating them with diamide, an oxidizing agent that specifically oxidizes thiol groups at indicated concentrations (125, 250 and 500 M)
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Inside cells, the enzyme glutathione reductase (using NADPH as a cofactor) continuously regenerates GSH from GSSG, maintaining a GSH:GSSG ratio of approximately 100:1 under healthy conditions
Cole SP, Bhardwaj G, Gerlach JH, Mackie JE, Grant CE, Almquist KC, Stewart AJ, Kurz EU, Duncan AM, Deeley RG
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