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Hosein et al., 2012) didnt display any toxicity profile related to renal dysfunction at dose-limiting toxicity (DLT)

To establish causality, C57BL/6J mice were randomized to receive either a high-cholesterol diet (HCD) or regular diet (RD) for three days prior to cecal ligation and puncture (CLP) to induce sepsis

If no validated condition is available, temporary storage of unopened lyophilized material at approximately 20C in a tightly sealed, dry, light-protected container may be used as a conservative laboratory holding condition, but it must not be presented as a validated shelf-life claim

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