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The PCR protocol included an initial denaturation at 95 C for 60 s, followed by 40 cycles of denaturation at 95 C for 15 s, annealing at 55 C for s, extension at 72 C for 35 s, and a final extension at 95 C for 30 s and 55 C for 35 s
In addition, OCTN2 protein was detected in exosomes released from HEK293 cells [166], indicating that carnitine and its derivatives may be transported between cells through exosomes, a finding that provides new insights into the role of carnitine and its transporters in exosomes and their functions in intercellular communication and pathological processes
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