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Missed doses follow

For reduced cholesterol, islets were pre-incubated in 10 M simvastatin (S6196, Sigma Aldrich) diluted in RPMI-1640 supplemented with 10% lipoprotein deficient serum (LPDS) (S5394, Sigma Aldrich) and 1% p/s for a minimum of 18 hours prior to the assays

It reflects the point at which benzyl alcohol's antimicrobial efficacy drops below sterile threshold and peptide aggregates begin forming even at refrigerated temperatures

The protocol (Supplementary Appendix A) was developed in accordance with Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) 2020 guidelines [6] and has been published on PROSPERO (ID: CRD420251051447)